Two-dimensional Crystallization and Preliminary Structure Analysis of Light Harvesting II (B800-850) Complex From the Purple Bacterium Rhodovulum sulfidophilum
Identifieur interne : 002D27 ( Main/Exploration ); précédent : 002D26; suivant : 002D28Two-dimensional Crystallization and Preliminary Structure Analysis of Light Harvesting II (B800-850) Complex From the Purple Bacterium Rhodovulum sulfidophilum
Auteurs : Guillermo Montoya [Allemagne] ; Marek Cyrklaff [Allemagne] ; Irmgard Sinning [Allemagne]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 1995.
English descriptors
- KwdEn :
- Teeft :
- Absorption spectrum, Analytical ultracentrifugation, Antenna complexes, Biophys, Boca raton, Carotenoid, Central hole, Circular dichroism, Cogdell, Communications figure, Crystallization, Crystallization conditions, Crystallization experiments, Different ratios, Dodecyl maltoside, Dose conditions, Double layer, Electron crystallography, Electron microscopy, Epon cell, Equilibration buffer, Febs letters, Free pigments, Image processing, Lattice, Lhii, Lhii complexes, Light energy, Light harvesting, Light harvesting complexes, Linear gradient, Lling height, Membrane, Membrane proteins, Methods enzymol, Molecular mass, Monoq column, Nacl, Octyl glucoside, Oligomerization state, Optical diffraction, Outer diameter, Photosynthetic, Photosynthetic bacteria, Photosynthetic membrane, Plane group, Preliminary structure analysis, Primary structure, Projection maps, Protein concentration, Purple photosynthetic bacteria, Reaction centre, Rhodopseudomonas, Rhodopseudomonas acidophila, Rhodospirillum, Rhodospirillum molischianum, Rhodospirillum rubrum, Right lane, Room temperature, Rubrum, Same buffer, Secondary structure, Sedimentation equilibrium, Spectral changes, Tetragonal lattice, Tubular crystals, Unit cells, Uranyl acetate, Vesicular crystals, Zuber.
Abstract
Abstract: Light-harvesting complex II (B800/850) from the purple bacteriumRhodovulum(Rhv.)sulfidophilumhas been isolated using a new protocal. It has been shown by analytical ultracentrifugation and native gels to be most likely an octamer. Two-dimensional crystals have been obtained by microdialysis. The plane group isp4212 witha=b= 157 Å. The crystals diffract to 18 Å in negative stain. Projection maps show clearly that LHII is organized in ring-like particles with an outer diameter of about 76 Å.f2 f2 Abbreviations used: LDAO, lauryl-dimethylamine-N-oxide; octyl-POE, octyl-pentaoxyethylene; C12E9, nonaethylene glycol monolauryl ether; 3D, three-dimensional; 2D, two-dimensional; LH, light harvesting; RC, reaction centre.
Url:
DOI: 10.1006/jmbi.1995.0353
Affiliations:
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uniqKey="Montoya G" first="Guillermo" last="Montoya">Guillermo Montoya</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg</wicri:regionArea><wicri:noRegion>69012 Heidelberg</wicri:noRegion><wicri:noRegion>69012 Heidelberg</wicri:noRegion></affiliation></author><author><name sortKey="Cyrklaff, Marek" sort="Cyrklaff, Marek" uniqKey="Cyrklaff M" first="Marek" last="Cyrklaff">Marek Cyrklaff</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg</wicri:regionArea><wicri:noRegion>69012 Heidelberg</wicri:noRegion><wicri:noRegion>69012 Heidelberg</wicri:noRegion></affiliation></author><author><name sortKey="Sinning, Irmgard" sort="Sinning, Irmgard" uniqKey="Sinning I" first="Irmgard" last="Sinning">Irmgard Sinning</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg</wicri:regionArea><wicri:noRegion>69012 Heidelberg</wicri:noRegion><wicri:noRegion>69012 Heidelberg</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Molecular Biology</title><title level="j" type="abbrev">YJMBI</title><idno type="ISSN">0022-2836</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">250</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="10">10</biblScope></imprint><idno type="ISSN">0022-2836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2836</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>B800-850</term><term>electron microscopy</term><term>membrane protein structure</term><term>photosynthesis</term><term>two-dimensional crystallization</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Absorption spectrum</term><term>Analytical ultracentrifugation</term><term>Antenna complexes</term><term>Biophys</term><term>Boca raton</term><term>Carotenoid</term><term>Central hole</term><term>Circular dichroism</term><term>Cogdell</term><term>Communications figure</term><term>Crystallization</term><term>Crystallization conditions</term><term>Crystallization experiments</term><term>Different ratios</term><term>Dodecyl maltoside</term><term>Dose conditions</term><term>Double layer</term><term>Electron crystallography</term><term>Electron microscopy</term><term>Epon cell</term><term>Equilibration buffer</term><term>Febs letters</term><term>Free pigments</term><term>Image processing</term><term>Lattice</term><term>Lhii</term><term>Lhii complexes</term><term>Light energy</term><term>Light harvesting</term><term>Light harvesting complexes</term><term>Linear gradient</term><term>Lling height</term><term>Membrane</term><term>Membrane proteins</term><term>Methods enzymol</term><term>Molecular mass</term><term>Monoq column</term><term>Nacl</term><term>Octyl glucoside</term><term>Oligomerization state</term><term>Optical diffraction</term><term>Outer diameter</term><term>Photosynthetic</term><term>Photosynthetic bacteria</term><term>Photosynthetic membrane</term><term>Plane group</term><term>Preliminary structure analysis</term><term>Primary structure</term><term>Projection maps</term><term>Protein concentration</term><term>Purple photosynthetic bacteria</term><term>Reaction centre</term><term>Rhodopseudomonas</term><term>Rhodopseudomonas acidophila</term><term>Rhodospirillum</term><term>Rhodospirillum molischianum</term><term>Rhodospirillum rubrum</term><term>Right lane</term><term>Room temperature</term><term>Rubrum</term><term>Same buffer</term><term>Secondary structure</term><term>Sedimentation equilibrium</term><term>Spectral changes</term><term>Tetragonal lattice</term><term>Tubular crystals</term><term>Unit cells</term><term>Uranyl acetate</term><term>Vesicular crystals</term><term>Zuber</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Light-harvesting complex II (B800/850) from the purple bacteriumRhodovulum(Rhv.)sulfidophilumhas been isolated using a new protocal. It has been shown by analytical ultracentrifugation and native gels to be most likely an octamer. Two-dimensional crystals have been obtained by microdialysis. The plane group isp4212 witha=b= 157 Å. The crystals diffract to 18 Å in negative stain. Projection maps show clearly that LHII is organized in ring-like particles with an outer diameter of about 76 Å.f2 f2 Abbreviations used: LDAO, lauryl-dimethylamine-N-oxide; octyl-POE, octyl-pentaoxyethylene; C12E9, nonaethylene glycol monolauryl ether; 3D, three-dimensional; 2D, two-dimensional; LH, light harvesting; RC, reaction centre.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Montoya, Guillermo" sort="Montoya, Guillermo" uniqKey="Montoya G" first="Guillermo" last="Montoya">Guillermo Montoya</name></noRegion><name sortKey="Cyrklaff, Marek" sort="Cyrklaff, Marek" uniqKey="Cyrklaff M" first="Marek" last="Cyrklaff">Marek Cyrklaff</name><name sortKey="Sinning, Irmgard" sort="Sinning, Irmgard" uniqKey="Sinning I" first="Irmgard" last="Sinning">Irmgard Sinning</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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